Format

Send to

Choose Destination
J Vis Exp. 2019 Nov 23;(153). doi: 10.3791/59905.

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria.

Author information

1
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida.
2
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida; eswara@usf.edu.

Abstract

Investigations of factors influencing cell division and cell shape in bacteria are commonly performed in conjunction with high-resolution fluorescence microscopy as observations made at a population level may not truly reflect what occurs at a single cell level. Live-cell timelapse microscopy allows investigators to monitor the changes in cell division or cell morphology which provide valuable insights regarding subcellular localization of proteins and timing of gene expression, as it happens, to potentially aid in answering important biological questions. Here, we describe our protocol to monitor phenotypic changes in Bacillus subtilis and Staphylococcus aureus using a high-resolution deconvolution microscope. The objective of this report is to provide a simple and clear protocol that can be adopted by other investigators interested in conducting fluorescence microscopy experiments to study different biological processes in bacteria as well as other organisms.

PMID:
31814606
DOI:
10.3791/59905

Supplemental Content

Full text links

Icon for MyJove Corporation
Loading ...
Support Center