Chemoproteomic profiling of protein-metabolite interactions

Wei Qin; Fan Yang; Chu Wang

doi:10.1016/j.cbpa.2019.11.003

Chemoproteomic profiling of protein-metabolite interactions

Curr Opin Chem Biol. 2020 Feb:54:28-36. doi: 10.1016/j.cbpa.2019.11.003. Epub 2019 Dec 5.

Authors

Wei Qin¹, Fan Yang², Chu Wang³

Affiliations

¹ Synthetic and Functional Biomolecules Center, Peking University, Beijing, China; Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
² Synthetic and Functional Biomolecules Center, Peking University, Beijing, China; Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, China; College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
³ Synthetic and Functional Biomolecules Center, Peking University, Beijing, China; Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China; Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China; College of Chemistry and Molecular Engineering, Peking University, Beijing, China. Electronic address: chuwang@pku.edu.cn.

PMID: 31812894
DOI: 10.1016/j.cbpa.2019.11.003

Abstract

Small molecule metabolites play important roles in regulating protein functions, which are acted through either covalent non-enzymatic post-translational modifications or non-covalent binding interactions. Chemical proteomic strategies can help delineate global landscapes of cellular protein-metabolite interactions and provide molecular insights about their mechanisms of action. In this review, we summarized the recent progress in developments and applications of chemoproteomic strategies to profile protein-metabolite interactions.

Keywords: Activity-based protein profiling; Chemoproteomics; Non-covalent binding; Post-translational modifications; Protein-metabolite interactions.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Humans
Metabolome / physiology*
Metabolomics / methods*
Proteins / chemistry*
Proteins / metabolism*
Proteome / chemistry*
Proteome / metabolism*
Proteomics / methods*

Substances

Proteins
Proteome