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Anticancer Res. 2019 Dec;39(12):6701-6709. doi: 10.21873/anticanres.13885.

Effects of Lyophilization of Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles on Intracellular Delivery.

Author information

1
Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, Japan.
2
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Osaka, Japan.
3
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
4
Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, Japan i-nakase@21c.osakafu-u.ac.jp.

Abstract

BACKGROUND/AIM:

Extracellular vesicles (exosomes, EVs) (30-200 nm in diameter) are secreted by various cells in the body. Owing to the pharmaceutical advantages of EVs, an EV-based drug delivery system (DDS) for cancer therapy is expected to be the next-generation therapeutic system. However, preservation methods for functional and therapeutic EVs should be developed. Here, we developed the method of lyophilization of arginine-rich cell penetrating peptide (CPP)-modified EVs and investigated the effects of lyophilization on the characteristics of EVs.

MATERIALS AND METHODS:

Particle size, structure, zeta-potential, and cellular uptake efficacy of the arginine-rich CPP-modified EVs were analyzed. The model protein saporin (SAP), having anti-cancer effects, was encapsulated inside the EVs to assess the cytosolic release of EV content after cellular uptake.

RESULTS:

Lyophilization of the EVs did not affect their particle size, structure, zeta-potential, and cellular uptake efficacy; however, the biological activity of the encapsulated SAP was inhibited by lyophilization.

CONCLUSION:

Lyophilization of EVs may affect SAP structures and/or reduce the cytosolic release efficacy of EV's content after cellular uptake and needs attention in EV-based DDSs.

KEYWORDS:

Extracellular vesicles; cell-penetrating peptides; exosomes; lyophilization; saporin

PMID:
31810935
DOI:
10.21873/anticanres.13885
[Indexed for MEDLINE]

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