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Cancers (Basel). 2019 Nov 29;11(12). pii: E1903. doi: 10.3390/cancers11121903.

WISP-1 Promotes Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells Via the miR-153-3p/Snail Axis.

Chang AC1, Lien MY2,3, Tsai MH1,4, Hua CH4, Tang CH1,5,6,7,8.

Author information

1
School and Medicine, China Medical University, Taichung 404, Taiwan.
2
Division of Hematology and Oncology, Department of Internal Medicine, China Medical University Hospital, Taichung 404, Taiwan.
3
Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan.
4
Department of Otolaryngology, China Medical University Hospital, Taichung 404, Taiwan.
5
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
6
Graduate Institute of Clinical Medical Science, China Medical University, Taichung 404, Taiwan.
7
Chinese Medicine Research Center, China Medical University, Taichung 404, Taiwan.
8
Department of Biotechnology, College of Health Science, Asia University, Taichung 413, Taiwan.

Abstract

Around half of all patients with oral squamous cell carcinoma (OSCC) present with lymphatic metastasis, a strong predictor of poor survival. Improving survival rates depends on preventing the first step in the "invasion-metastasis cascade," epithelial-to-mesenchymal transition (EMT), and developing antilymphangiogenesis therapies that antagonize lymphatic metastasis. The extracellular matrix-related protein WISP-1 (WNT1-inducible signaling pathway protein-1) stimulates bone remodeling and tumor progression. We have previously reported that WISP-1 promotes OSCC cell migration and lymphangiogenesis induced by vascular endothelial growth factor C (VEGF-C). This investigation sought to determine the role of WISP-1 in regulating EMT in OSCC. Our analysis of oral cancer data from The Cancer Genome Atlas (TCGA) database revealed significant and positive associations between levels of WISP-1 expression and clinical disease stage, as well as regional lymph node metastasis. We also found higher levels of WISP-1 expression in serum samples obtained from patients with OSCC compared with samples from healthy controls. In a series of in vitro investigations, WISP-1 activated EMT signaling via the FAK/ILK/Akt and Snail signaling transduction pathways and downregulated miR-153-3p expression in OSCC cells. Our findings detail how WISP-1 promotes EMT via the miR-153-3p/Snail axis in OSCC cells.

KEYWORDS:

EMT; OSCC; Snail; WISP-1; miR-153-3p

PMID:
31795469
DOI:
10.3390/cancers11121903
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