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Stem Cell Res. 2019 Nov 23;42:101663. doi: 10.1016/j.scr.2019.101663. [Epub ahead of print]

A collection of three integration-free iPSCs derived from old male and female healthy subjects.

Author information

1
Instituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain.
2
Servicio de Bioquímica Clínica, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
3
Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain; Movement Disorders Unit, Neurology Dept., Hospital Sant Pau, Barcelona, Spain.
4
Instituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain. Electronic address: cvicario@cajal.csic.es.

Abstract

Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.

PMID:
31794941
DOI:
10.1016/j.scr.2019.101663
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