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Methods Mol Biol. 2020;2092:1-18. doi: 10.1007/978-1-0716-0175-4_1.

Investigating Neurogenesis in Birds.

Author information

1
Department of Molecular Biology, Sciences III, University of Geneva, Geneva, Switzerland.
2
Department of Biochemistry, Sciences II, University of Geneva, Geneva, Switzerland.
3
Department of Molecular Biology, Sciences III, University of Geneva, Geneva, Switzerland. Jean-Marc.Matter@unige.ch.
4
Department of Biochemistry, Sciences II, University of Geneva, Geneva, Switzerland. Jean-Marc.Matter@unige.ch.

Abstract

The macula and fovea make human vision unique among mammals. An understanding of the genetic network underlying the development and maintenance of this highly specialized region is instrumental to address issues about human macula-related retinopathies. The pigeon retina, unlike currently available animal models, shares numerous key characteristics of the primate macula and represents a promising new model for the study of retinal development. We provide key elements to take advantage of this new model for the study of retina and brain development. This includes precise embryo staging, transfection of genetic material (reporter plasmid, expression vectors, siRNAs) using in ovo and ex vivo electroporation, live imaging, high-resolution confocal imaging, and data layout and instructions for data analysis.

KEYWORDS:

Avian; Brain; Chick; Development; Electroporation; Live imaging; Optic tectum; Pigeon; Retina; Time-lapse imaging

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