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Sci Total Environ. 2019 Nov 23:135542. doi: 10.1016/j.scitotenv.2019.135542. [Epub ahead of print]

High-throughput non-targeted metabolomics study of the effects of perfluorooctane sulfonate (PFOS) on the metabolic characteristics of A. thaliana leaves.

Author information

1
Key Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China.
2
Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Shanghai, China.
3
Key Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China. Electronic address: zhangyanwei14@163.com.

Abstract

The ecotoxicity of perfluorooctane sulfonate (PFOS) is complex and has been reported in animals (including fish and mice), but the effects of PFOS in plants, especially the toxic mechanisms, have rarely been studied. High-throughput nontargeted metabolomics methods for comprehensive assessment were selected to study changes in metabolic characteristics in Arabidopsis thaliana leaves by exposure to different concentrations of PFOS throughout the growth period (30 days). All the metabolites were analyzed by PCA and OPLS-DA methods, by the cutoff of VIP and p-value, 53 biomarkers were found and significantly regulated, all amino acids except glutamate were inhibited and probably associated with binding to protein, auxin and cytokinin of phytohormones were significantly down-regulated. In response mechanism to oxidative stress from PFOS, the phenylpropanoid pathway were fully activated to form several polyphenols and further enhanced into several flavonoids against the reactive oxygen species (ROS) as the primary defend pathway, in addition, ascorbate, trehalose and nicotinamide also were activated and help decrease the damage from oxidative stress. These results provide insights into the mechanism underlying the phytotoxicity of PFOS.

KEYWORDS:

Metabolic mechanism; Metabolomics; PFOS; Phytotoxicity

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