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Kidney Int. 2020 Mar;97(3):609-614. doi: 10.1016/j.kint.2019.08.029. Epub 2019 Sep 18.

A collagen-binding protein enables molecular imaging of kidney fibrosis in vivo.

Author information

1
Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, Aachen, Germany.
2
Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany; Department of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany.
3
Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, Aachen, Germany; Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany.
4
Department of Biochemistry, University Hospital Maastricht (azM), Maastricht, The Netherlands.
5
Department of Pathology, Electron Microscopy Unit, University of Maastricht, Maastricht, The Netherlands.
6
Institute of Pathology, University Erlangen, Erlangen, Germany.
7
Department of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany; Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia; Department of Clinical Medicine, University of Bergen, Bergen, Norway.
8
Department of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany.
9
Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, Aachen, Germany; Department of Pharmaceutics, Utrecht University, Utrecht, The Netherlands; Department of Targeted Therapeutics, University of Twente, Enschede, The Netherlands. Electronic address: tlammers@ukaachen.de.
10
Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany; Department of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany; Electron Microscopy Facility, RWTH Aachen University Hospital, Aachen, Germany. Electronic address: pboor@ukaachen.de.

Abstract

Pathological deposition of collagen is a hallmark of kidney fibrosis. To illustrate this process we employed multimodal optical imaging to visualize and quantify collagen deposition in murine models of kidney fibrosis (ischemia-reperfusion or unilateral ureteral obstruction) using the collagen-binding adhesion protein CNA35. For in vivo imaging, we used hybrid computed tomography-fluorescence molecular tomography and CNA35 labeled with the near-infrared fluorophore Cy7. Upon intravenous injection, CNA35-Cy7 accumulation was significantly higher in fibrotic compared to non-fibrotic kidneys. This difference was not detected for a non-specific scrambled version of CNA35-Cy7. Ex vivo, on kidney sections of mice and patients with renal fibrosis, CNA35-FITC co-localized with fibrotic collagen type I and III, but not with the basement membrane collagen type IV. Following intravenous injection, CNA35-FITC bound to both interstitial and perivascular fibrotic areas. In line with this perivascular accumulation, we observed significant perivascular fibrosis in the mouse models and in biopsy sections from patients with chronic kidney disease using computer-based morphometry quantification. Thus, molecular imaging of collagen using CNA35 enabled specific non-invasive quantification of kidney fibrosis. Collagen imaging revealed significant perivascular fibrosis as a consistent component next to the more commonly assessed interstitial fibrosis. Our results lay the basis for further probe and protocol optimization towards the clinical translation of molecular imaging of kidney fibrosis.

KEYWORDS:

chronic kidney disease (CKD); collagen; extracellular matrix; molecular imaging; non-invasive imaging; renal fibrosis

PMID:
31784048
DOI:
10.1016/j.kint.2019.08.029

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