Don't mess with the machine - evaluation of fluconazole susceptibility testing for Candida glabrata using the new VITEK2 AST-YS08 card following species modification

Maya Korem; Yuval Geffen; Sharon Amit

doi:10.1016/j.diagmicrobio.2019.114896

Don't mess with the machine - evaluation of fluconazole susceptibility testing for Candida glabrata using the new VITEK2 AST-YS08 card following species modification

Diagn Microbiol Infect Dis. 2020 Feb;96(2):114896. doi: 10.1016/j.diagmicrobio.2019.114896. Epub 2019 Sep 13.

Authors

Maya Korem¹, Yuval Geffen², Sharon Amit³

Affiliations

¹ Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
² Clinical Microbiology Laboratory, Rambam Medical Center, Haifa, Israel.
³ Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel. Electronic address: sharonamit@hadassah.org.il.

PMID: 31780195
DOI: 10.1016/j.diagmicrobio.2019.114896

Abstract

A modification of fluconazole formulation in the VITEK2 AST-YS08 card revoked the fluconazole-Candida glabrata combination. An evaluation of AST-YS08 following C. glabrata to C. albicans adjustment within VITEK2 software revealed higher fluconazole MICs compared to AST-YS07 and E-test, with major discrepancies. This mandates an alternative approach to fluconazole-C. glabrata susceptibility testing.

Keywords: AST-YS08 card; Candida glabrata; Susceptibility testing; VITEK2.

MeSH terms

Antifungal Agents / pharmacology*
Candida glabrata / drug effects*
Candidiasis / diagnosis
Candidiasis / drug therapy
Candidiasis / microbiology
Fluconazole / pharmacology*
Humans
Microbial Sensitivity Tests* / methods
Microbial Sensitivity Tests* / standards
Reagent Kits, Diagnostic* / standards
Reproducibility of Results

Substances

Antifungal Agents
Reagent Kits, Diagnostic
Fluconazole