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Biotechniques. 2019 Nov 29. doi: 10.2144/btn-2019-0092. [Epub ahead of print]

Methodology for the at-home collection of urine samples for prostate cancer detection.

Author information

1
Norwich Medical School, University of East Anglia, Norwich, UK.
2
Norfolk & Norwich University Hospitals NHS Foundation Trust, Norwich, UK.
3
Group of Biomedical Research in Urology, Vall d'Hebron Research Institute & Universitat Autònoma de Barcelona, Barcelona, Spain.
4
Prostate Cancer Research Group, Centre for Molecular Medicine Norway, University of Oslo & Oslo University Hospital, Oslo, Norway.
5
Department of Tumour Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.
6
School of Medicine, Dentistry & Biomedical Sciences, Institute for Health Sciences, Centre for Cancer Research & Cell Biology, Queen's University Belfast, Belfast, UK.
7
Earlham Institute, Norwich, UK.

Abstract

Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic.

KEYWORDS:

biomarker; cancer; diagnosis; home-screening; prognosis; prostate; urine

PMID:
31779479
DOI:
10.2144/btn-2019-0092

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