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Virol Sin. 2019 Nov 27. doi: 10.1007/s12250-019-00170-9. [Epub ahead of print]

Analysis of Expression Profiles of Long Noncoding RNAs and mRNAs in A549 Cells Infected with H3N2 Swine Influenza Virus by RNA Sequencing.

Author information

1
MOA Key Laboratory of Animal Virology and Department of Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.
2
MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety, Institute of Immunology and College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
3
MOA Key Laboratory of Animal Virology and Department of Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. jyzhou@zju.edu.cn.

Abstract

Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in influenza A virus (IAV) pathogenicity are largely unknown. Here, we analyzed the expression profiles of lncRNAs and mRNAs in H3N2-infected cells and mock-infected cells by high-throughput sequencing. The results showed that 6129 lncRNAs and 50,031 mRNA transcripts in A549 cells displayed differential expression after H3N2 infection compared with mock infection. Among the differentially expressed lncRNAs, 4963 were upregulated, and 1166 were downregulated. Functional annotation and enrichment analysis using gene ontology and Kyoto Encyclopedia of Genes and Genomes databases (KEGG) suggested that target genes of the differentially expressed lncRNAs were enriched in some biological processes, such as cellular metabolism and autophagy. The up- or downregulated lncRNAs were selected and further verified by quantitative real-time polymerase chain reaction (RT-qPCR) and reverse transcription PCR (RT-PCR). To the best of our knowledge, this is the first report of a comparative expression analysis of lncRNAs in A549 cells infected with H3N2. Our results support the need for further analyses of the functions of differentially expressed lncRNAs during H3N2 infection.

KEYWORDS:

A549 cells; High-throughput sequencing; Influenza virus (IAV); Long noncoding RNA (lncRNA)

PMID:
31777011
DOI:
10.1007/s12250-019-00170-9

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