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Stem Cell Res. 2019 Oct 25;41:101610. doi: 10.1016/j.scr.2019.101610. [Epub ahead of print]

Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing.

Author information

1
Department of Obstetrics, Xiangya Hospital Central South University, 87 Xiangya Road, Changsha 410008, China.
2
Department of Obstetrics, Xiangya Hospital Central South University, 87 Xiangya Road, Changsha 410008, China; Hunan Engineering Research Center of Early Life Development and Disease Prevention, 87 Xiangya Road, Changsha 410008, China. Electronic address: 1471674914@qq.com.

Abstract

Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential.

PMID:
31775087
DOI:
10.1016/j.scr.2019.101610
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