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J Vis Exp. 2019 Nov 8;(153). doi: 10.3791/60292.

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery.

Author information

1
Department of Microsurgery, Jesús Usón Minimally Invasive Surgery Centre; aballestin@ccmijesususon.com.
2
Stem Cell Therapy Unit, Jesús Usón Minimally Invasive Surgery Centre.
3
Department of Microsurgery, Jesús Usón Minimally Invasive Surgery Centre.
4
Animal Modelling Unit, Jesús Usón Minimally Invasive Surgery Centre.
5
Department of Microsurgery, Jesús Usón Minimally Invasive Surgery Centre; Stem Cell Therapy Unit, Jesús Usón Minimally Invasive Surgery Centre; Animal Modelling Unit, Jesús Usón Minimally Invasive Surgery Centre.

Abstract

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many areas of biomedical research due to its cost-effectiveness and its translation to humans. This protocol describes a method to create a preclinical free skin flap model in rats with ischemia-reperfusion injury. The described 3 cm x 6 cm rat free skin flap model is easily obtained after the placement of several vascular ligatures and the section of the vascular pedicle. Then, 8 h after the ischemic insult and completion of the microsurgical anastomosis, the free skin flap develops the tissue damage. These ischemia-reperfusion injury-related damages can be studied in this model, making it a suitable model for evaluating therapeutic agents to address this pathophysiological process. Furthermore, two main monitoring techniques are described in the protocol for the assessment of this animal model: transit-time ultrasound technology and laser speckle contrast analysis.

PMID:
31762459
DOI:
10.3791/60292

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