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Anal Chim Acta. 2020 Jan 15;1094:142-150. doi: 10.1016/j.aca.2019.10.012. Epub 2019 Oct 12.

Gold-nanourchin seeded single-walled carbon nanotube on voltammetry sensor for diagnosing neurogenerative Parkinson's disease.

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Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, No.324, Jingwu Road, Huaiyin District, Jinan, Shandong, 250021, PR China.
Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250021, PR China.
Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia. Electronic address:
Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University, Jinan, 250001, Shandong, China; Department of Neurosurgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No.92, Aiguo Road, Nanchang, Jiangxi, 330006, China. Electronic address:


α-synuclein is a predominantly expressing neuronal protein for understanding the neurodegenerative disorders. A diagnosing system with aggregated α-synuclein encoded by SNCA gene is necessary to make the precautionary treatment against Parkinson's disease (PD). Herein, gold-nanourchin conjugated anti-α-synuclein antibody was desired as the probe and seeded on single-walled carbon nanotube (SWCN) integrated interdigitated electrode (IDE). The surface morphology of SWCN-modified IDE and gold urchin-antibody conjugates were observed under FESEM, FETEM and AFM, the existing elements were confirmed. Voltammetry analysis revealed that the limit of fibril-formed α-synuclein detection was improved by 1000 folds (1 fM) with gold-nanourchin-antibody modified surface, compared to the surface with only antibody (1 pM). Validating the interaction of α-synuclein by Enzyme-linked Immunosorbent Assay was displayed the detection limit as 10 pM. IDE has a good reproducibility and a higher selectivity on α-synuclein as evidenced by the interactive analysis with the control proteins, PARK1 and DJ-1.


Fibril-formed α-synuclein; Gold-nanocomposite; Interdigitated electrode; Prodromal parkinsonism; Single-walled carbon nanotube


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