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Teratology. 1988 Aug;38(2):181-5.

Application of Y chromosomal repetitive sequences to sexing mouse embryos.

Author information

1
Department of Biology, McGill University, Montreal, Quebec, Canada.

Abstract

Identification of sex is often necessary to evaluate genetic or teratogenetic effects on embryonic development. A simple molecular technique to identify the sex of mouse embryos was studied using a Y chromosomal repetitive sequence (designated 145SC5). Since this technique does not require purification of DNA, it is particularly suitable for processing many embryos. Furthermore, 145SC5 detects 1% contamination of male DNA in a male-female DNA mixture. These results suggest that 145SC5 is a powerful molecular tool in a variety of studies on mouse development.

PMID:
3175952
DOI:
10.1002/tera.1420380211
[Indexed for MEDLINE]

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