Format

Send to

Choose Destination
Arch Virol. 2019 Nov 19. doi: 10.1007/s00705-019-04465-w. [Epub ahead of print]

Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase.

Author information

1
State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation. evseenko_va@vector.nsc.ru.
2
State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation.

Abstract

Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT50) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT50 was observed with the seasonal human H3N2 virus (45.5 °C). The IT50 values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca2+ binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.

PMID:
31745716
DOI:
10.1007/s00705-019-04465-w

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center