Format

Send to

Choose Destination
Indian J Dent Res. 2019 Jul-Aug;30(4):595-599. doi: 10.4103/ijdr.IJDR_67_16.

A crude method of DNA extraction and identification from exfoliated human buccal mucosa cells.

Author information

1
Department of Oral Pathology and Microbiology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
2
Department of Oral Pathology and Microbiology, ITS Dental College, Greater Noida, Uttar Pradesh, India.

Abstract

Background:

DNA analysis has a key role in forensic dentistry. However, techniques of DNA extraction and analysis are far from the reach of majority of medical professionals owing to its expensive set up.

Aim:

The present study was aimed at formulating a crude method of extracting DNA from human buccal mucosa cells using materials commonly available in the laboratory so that the medical professionals could get more exposure to molecular biology techniques. The objectives were to identify the DNA and to assess its purity.

Methods:

Buccal mucosa cells from 10 healthy volunteers were taken for DNA extraction following the protocol of cell lysis, purification, and precipitation. DNA was identified using standardized techniques like Diphenylamine test and its purity was assessed using a spectrophotometer. A gel electrophoresis apparatus was also constructed using readily available materials.

Results:

DNA was extracted from human buccal mucosa cells using a crude method. The standardized tests confirmed the presence of DNA contaminated with proteins. The locally made Gel electrophoresis model exhibited a faint halo around the wells instead of DNA bands.

Conclusion:

DNA extraction from human buccal mucosa cells was made possible using locally available materials and a crude method, but it was not of high purity.

KEYWORDS:

Buccal mucosa; DNA extraction; gel electrophoresis; molecular biology; spectrophotometer

PMID:
31745059
DOI:
10.4103/ijdr.IJDR_67_16
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Medknow Publications and Media Pvt Ltd
Loading ...
Support Center