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Stem Cell Res. 2019 Nov 6;41:101644. doi: 10.1016/j.scr.2019.101644. [Epub ahead of print]

Generation of two ERF gene knockout human embryonic stem cell lines using CRISPR/Cas9 system.

Author information

1
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, China.
2
Meihekou Central Hospital, China.
3
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, China. Electronic address: iamxuyan@sohu.com.

Abstract

Human ERF gene is a transcription factor involved in development, trophoblast differentiation, apoptosis, and cancer progress. To further understand the exact roles of ERF in these processes, here we report that establishment of two ERF knockout human embryonic stem cell (hESC) lines by CRISPR/Cas9 mediated gene targeting. These cell lines exhibited classical hESC morphology and normal karyotype, and highly expressed pluripotent markers, and had differentiation potential in vitro. These cell lines provide good materials to understand the roles of ERF in development, trophoblast differentiation and craniosynostosis for further studies.

PMID:
31743839
DOI:
10.1016/j.scr.2019.101644
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