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PLoS One. 2019 Nov 18;14(11):e0225249. doi: 10.1371/journal.pone.0225249. eCollection 2019.

Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass.

Author information

1
Department of Pathology, Herlev Hospital, University of Copenhagen, Herlev, Denmark.
2
Department of Autoimmunology and Biomarkers, Statens Serum Institut, Copenhagen, Denmark.
3
Department of Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark.
4
Department of Gynaecology, Juliane Marie Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

Abstract

BACKGROUND:

Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses.

METHODS:

The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery group and a validation group, each consisting of 190 pre-surgery plasma samples from age-matched patients with malignant (n = 95) and benign pelvic mass (n = 95), by real time RT-qPCR.

RESULTS:

Four up-regulated (miR-200c-3p, miR-221-3p, miR-21-5p, and miR-484) and two down-regulated (miR-195-5p and miR-451a) microRNAs were discovered. From those, miR-200c-3p and miR-221-3p were further confirmed in a validation cohort. A combination of these 2 microRNAs together with CA-125 yielded an overall diagnostic accuracy of AUC = 0.96.

CONCLUSIONS:

We showed consistent plasma microRNA profiles that provide independent diagnostic information of late stage OC.

PMID:
31738788
DOI:
10.1371/journal.pone.0225249
Free PMC Article

Conflict of interest statement

The authors have declared that no competing interests exist.

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