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Ann Indian Acad Neurol. 2019 Oct-Dec;22(4):474-476. doi: 10.4103/aian.AIAN_515_18. Epub 2019 Oct 25.

Interleukin 18 Polymorphisms and its serum level in Patients with Multiple Sclerosis.

Author information

1
Isfahan Medical Students Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
2
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3
Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
4
Department of Ophthalmology, Feiz Eye Hospital, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

Background:

Multiple sclerosis (MS) is a chronic demyelinating disorder of central nervous system. Although the definite pathogenesis of MS has not been understood, crucial role of environmental and genetic risk factors has been proposed.

Propose:

To determine the serum level of interleukin-18 (IL-18) as well as gene polymorphisms of IL-18 (rs1946518, rs360719, and rs187238).

Methods:

In this case-control study, 110 MS patients diagnosed according to the McDonald criteria and 110 healthy individuals were recruited. IL-18 gene polymorphisms were genotyped by polymerase chain reaction high-resolution melt test, and IL-18 serum level was determined by enzyme-linked immunosorbent assay technique.

Results:

The mean age of the MS patients (89 females and 21 males) and the control group (89 females and 21 males) was 30.3 ± 9.25 and 30.28 ± 9.13 years, respectively. The mean serum levels of IL-18 in MS patients and healthy individuals were 341.56 ± 39.22 Pg/Ml and 146.52 ± 29.30 Pg/Ml, respectively (P < 0.001). The genotype of rs1946518 (but not rs360719 and rs187238) was significantly different between groups (P = 0.037 and P = 0.069, respectively).

Conclusion:

In this study, we showed the significant higher IL-18 serum level and significant different frequencies of two polymorphisms of IL-18 in MS patients. These results show the important roles of IL-18 in MS pathogenesis. However, more studies are needed to verify our results in larger sample size.

KEYWORDS:

Multiple sclerosis; interleukin 18; serum level; single-nucleotide polymorphism

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