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Stem Cell Res. 2019 Nov 8;41:101654. doi: 10.1016/j.scr.2019.101654. [Epub ahead of print]

Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins.

Author information

1
LUMC human iPSC Hotel, Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, 2333 ZC, the Netherlands.
2
Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, PO Box 9502, 2300 RA, Leiden, the Netherlands.
3
Department of Biological Psychology, VU University Amsterdam, Neuroscience Amsterdam, Amsterdam, the Netherlands.
4
LUMC human iPSC Hotel, Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, 2333 ZC, the Netherlands. Electronic address: k.i.raymond@lumc.nl.

Abstract

Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing.

PMID:
31734644
DOI:
10.1016/j.scr.2019.101654
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