Format

Send to

Choose Destination
Cell Host Microbe. 2019 Nov 13;26(5):691-701.e5. doi: 10.1016/j.chom.2019.10.016.

A Listeria monocytogenes Bacteriocin Can Target the Commensal Prevotella copri and Modulate Intestinal Infection.

Author information

1
Institut Pasteur, Unité des Interactions Bactéries-Cellules, 75015 Paris, France; Inserm, U604, 75015 Paris, France; INRA, Unité sous-contrat 2020, 75015 Paris, France.
2
Neurosciences Institute, Georgia State University (GSU), Atlanta, GA 30303, USA; Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, GSU, Atlanta, GA 30303, USA.
3
Center of Microbial Ecology and Technology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
4
Institut Pasteur, Unité Biologie des Infections, 75015 Paris, France; Inserm, U1117, 75015 Paris, France.
5
Institut Pasteur, Unité Biologie des Infections, 75015 Paris, France; Inserm, U1117, 75015 Paris, France; Paris Descartes University, Sorbonne Paris Cité, Division of Infectious Diseases and Tropical Medicine, Necker-Enfants Malades University Hospital, Institut Imagine, 75743 Paris, France.
6
Institut Pasteur, Unité des Interactions Bactéries-Cellules, 75015 Paris, France; Inserm, U604, 75015 Paris, France; INRA, Unité sous-contrat 2020, 75015 Paris, France; Université de Paris, 75013 Paris, France.
7
Animalerie Centrale, Department of Technology and Scientific Programmes, Institut Pasteur, 75015 Paris, France.
8
The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.
9
The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA; Howard Hughes Medical Institute, New York, NY 10016, USA.
10
Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, GSU, Atlanta, GA 30303, USA.
11
Institut Pasteur, Unité des Interactions Bactéries-Cellules, 75015 Paris, France; Inserm, U604, 75015 Paris, France; INRA, Unité sous-contrat 2020, 75015 Paris, France. Electronic address: pascale.cossart@pasteur.fr.

Abstract

Understanding the role of the microbiota components in either preventing or favoring enteric infections is critical. Here, we report the discovery of a Listeria bacteriocin, Lmo2776, which limits Listeria intestinal colonization. Oral infection of conventional mice with a Δlmo2776 mutant leads to a thinner intestinal mucus layer and higher Listeria loads both in the intestinal content and deeper tissues compared to WT Listeria. This latter difference is microbiota dependent, as it is not observed in germ-free mice. Strikingly, it is phenocopied by pre-colonization of germ-free mice before Listeria infection with Prevotella copri, an abundant gut-commensal bacteria, but not with the other commensals tested. We further show that Lmo2776 targets P. copri and reduces its abundance. Together, these data unveil a role for P.copri in exacerbating intestinal infection, highlighting that pathogens such as Listeria may selectively deplete microbiota bacterial species to avoid excessive inflammation.

KEYWORDS:

Bacteriocin; Listeria monocytogenes; Lmo2776; M-SHIME; Prevotella copri; infection; microbiota

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center