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Microsurgery. 2019 Nov 14. doi: 10.1002/micr.30533. [Epub ahead of print]

A scaffold-free Bio 3D nerve conduit for repair of a 10-mm peripheral nerve defect in the rats.

Author information

1
Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
2
Department of Physical Therapy, Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
3
Cyfuse Biomedical, K.K., Tokyo, Japan.
4
Department of Regenerative Medicine and Biomedical Engineering Faculty of Medicine, Saga University, Saga, Japan.

Abstract

INTRODUCTION:

A Bio 3D printed nerve conduit was reported to promote nerve regeneration in a 5 mm nerve gap model. The purpose of this study was to fabricate Bio 3D nerve conduits suitable for a 10 mm nerve gap and to evaluate their capacity for nerve regeneration in a rat sciatic nerve defect model.

MATERIALS AND METHODS:

Eighteen F344 rats with immune deficiency (9-10 weeks old; weight, 200-250 g) were divided into three groups: a Bio 3D nerve conduit group (Bio 3D, n = 6), a nerve graft group (NG, n = 6), and a silicon tube group (ST, n = 6). A 12-mm Bio 3D nerve conduit or silicon tube was transplanted into the 10-mm defect of the right sciatic nerve. In the nerve graft group, reverse autografting was performed with an excised 10-mm nerve segment. Assessments were performed at 8 weeks after the surgery.

RESULTS:

In the region distal to the suture site, the number of myelinated axons in the Bio 3D group were significantly larger compared with the silicon group (2,548 vs. 950, p < .05). The myelinated axon diameter (MAD) and the myelin thickness (MT) of the regenerated axons in the Bio 3D group were significantly larger compared with those of the ST group (MAD: 3.09 vs. 2.36 μm; p < .01; MT: 0.59 vs. 0.40 μm, p < .01).

CONCLUSIONS:

This study indicates that a Bio 3D nerve conduit can enhance peripheral nerve regeneration even in a 10 mm nerve defect model.

PMID:
31724780
DOI:
10.1002/micr.30533

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