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Food Chem Toxicol. 2019 Nov 8:110957. doi: 10.1016/j.fct.2019.110957. [Epub ahead of print]

Correlation between 3-MCPD-induced organ toxicity and oxidative stress response in male mice.

Author information

1
German Federal Institute for Risk Assessment, Department of Food Safety, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.
2
School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, United Kingdom.
3
German Federal Institute for Risk Assessment, Department of Food Safety, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany. Electronic address: thorsten.buhrke@bfr.bund.de.

Abstract

3-Chloro-1,2-propanediol (3-MCPD) is a food contaminant which has been classified as a non-genotoxic carcinogen (category 2B). Previous studies suggested that oxidative stress might play a role in 3-MCPD toxicity. To elucidate the impact of 3-MCPD-mediated organ toxicity in more detail, transgenic reporter mice were employed which contain a lacZ reporter under the control of the heme oxygenase 1 (Hmox1) promoter which is responsive to oxidative stress. The mice received daily doses of up to 100 mg/kg body weight 3-MCPD per day in a 28-day feeding study. Subsequently, tissue slices from different organs were subjected to X-Gal staining as the readout for lacZ gene expression. A dose-dependent increase of blue stain was observed in mouse kidney that was exclusively visible in the renal cortex but not in the renal medulla. Moreover, blue-stained regions were detected in the basal membrane of the seminiferous tubules in testes and also in specific brain regions (cerebellum, midbrain and pons). Notably, gene expression of a number of Nrf2-dependent target genes except Hmox1 was not severely affected by 3-MCPD. In all three organs, however, the amount of irreversibly oxidized DJ-1 protein, which is a biomarker for oxidative stress, was significantly increased already by low doses of 3-MCPD.

KEYWORDS:

3-MCPD; Hmox1 activation; Organ toxicity; Oxidative stress

PMID:
31712104
DOI:
10.1016/j.fct.2019.110957

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