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Stem Cell Res. 2019 Oct 21;41:101630. doi: 10.1016/j.scr.2019.101630. [Epub ahead of print]

Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.

Author information

1
Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.
2
Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.
3
Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.
4
Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, RJ, Brazil.
5
Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, RJ, Brazil. Electronic address: bruno.solano@fiocruz.br.

Abstract

Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro.

PMID:
31706097
DOI:
10.1016/j.scr.2019.101630
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