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Anticancer Res. 2019 Nov;39(11):5911-5918. doi: 10.21873/anticanres.13795.

Double-negative T Cells Inhibit Proliferation and Invasion of Human Pancreatic Cancer Cells in Co-culture.

Author information

1
Department of General Surgery, Medical School of Shandong University, Jinan, P.R. China.
2
Department of General Surgery, Anhui Provincial Hospital, Hefei, P.R. China.
3
Origin Biosciences Inc., Nanjing, P.R. China.
4
Department of Surgery, University of California, San Diego, CA, U.S.A.
5
Anticancer Inc, San Diego, CA, U.S.A.
6
Department of General Surgery, Anhui Provincial Hospital, Hefei, P.R. China ch_jiong@126.com.

Abstract

BACKGROUND/AIM:

Double-negative T (DNT) cells are phenotypically CD3+CD4-CD8-T cells. This study aimed to investigate the anti-cancer activity of DNT cells against pancreatic cancer cells.

MATERIALS AND METHODS:

DNT cells were isolated from human peripheral blood. The effect of DNT cells on proliferation and invasion of the human pancreatic cell line Panc-1 was assessed. Expression of Nrf2 and Fas in Panc-1 cells co-cultured with DNT cells was analyzed with RT-PCR. The supernatants of Panc-1 and DNT co-cultures were analyzed with ELISA for IFN-r and FasL levels.

RESULTS:

The isolated DNT cell phenotype was CD4-CD8-CD56- CD3+TCR (T cell receptor) α/β+ T cells with more than 90% purity. Panc-1 cell proliferation was significantly inhibited by co-culture with DNT cells. Panc-1 cells co-cultured with DNT cells showed significantly reduced cell invasion. Panc-1 cells co-cultured with DNT cells showed increased Nrf2 and Fas mRNA expression. Increased INF-r and FasL levels were detected in the supernatants of co-cultures of DNT and pancreatic cells.

CONCLUSION:

DNT cells inhibited proliferation and invasion of human pancreatic cancer cells. The INF-r, Fas/FasL pathway and Nrf2 may be involved in the anti-cancer effect of DNT cells against human pancreatic cancer.

KEYWORDS:

Double-negative T cell; Nrf-2; cell proliferation invasion; pancreatic cancer

PMID:
31704815
DOI:
10.21873/anticanres.13795
[Indexed for MEDLINE]

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