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Stem Cell Res. 2019 Oct 19;41:101615. doi: 10.1016/j.scr.2019.101615. [Epub ahead of print]

Generation of four integration-free iPSC lines from related human donors.

Author information

1
TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Germany. Electronic address: anja.ramme@tissuse.com.
2
TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Germany.

Abstract

Four integration-free iPSC lines were generated by reprogramming peripheral blood mononuclear cells with episomal vectors. All four human donors (two male and two female donors) belong to one Caucasian family within three different generations with the age between 19-82 years. Additionally, all iPSC lines are approved for commercial use by donor consent. Those iPSC lines offer the opportunity to study the influence of affiliation within one family. In future, more iPSCs lines of many more family members can be created to understand the effects of relatives with different ages on the reprogramming into iPSCs and differentiation into specific cell types.

KEYWORDS:

Differentiation; Human; Induced pluripotent stem cells; Reprogramming; iPSCs

PMID:
31704539
DOI:
10.1016/j.scr.2019.101615
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