Topography and pachymetry maps for mouse corneas using optical coherence tomography

Alice S Liu; Dillon M Brown; Rachel E Conn; Ryan P McNabb; Machelle T Pardue; Anthony N Kuo

doi:10.1016/j.exer.2019.107868

Topography and pachymetry maps for mouse corneas using optical coherence tomography

Exp Eye Res. 2020 Jan:190:107868. doi: 10.1016/j.exer.2019.107868. Epub 2019 Nov 5.

Authors

Alice S Liu¹, Dillon M Brown², Rachel E Conn³, Ryan P McNabb⁴, Machelle T Pardue⁵, Anthony N Kuo⁶

Affiliations

¹ Ophthalmology, Duke University, Durham, NC, USA. Electronic address: sl332@duke.edu.
² Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
³ Neuroscience, Emory University, Atlanta, GA, USA.
⁴ Ophthalmology, Duke University, Durham, NC, USA.
⁵ Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA; Neuroscience, Emory University, Atlanta, GA, USA; Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Healthcare System, Decatur, GA, USA.
⁶ Ophthalmology, Duke University, Durham, NC, USA; Biomedical Engineering, Duke University, Durham, NC, USA. Electronic address: anthony.kuo@duke.edu.

Abstract

The majority of the eye's refractive power lies in the cornea, and pathological changes in its shape can affect vision. Small animal models offer an unparalleled degree of control over genetic and environmental factors that can help elucidate mechanisms of diseases affecting corneal shape. However, there is not currently a method to characterize the corneal shape of small animal eyes with topography or pachymetry maps, as is done clinically for humans. We bridge this gap by demonstrating methods using optical coherence tomography (OCT) to generate the first topography and pachymetry (thickness) maps of mouse corneas. Radii of curvature acquired using OCT were validated using calibration spheres as well as in vivo mouse corneas with a mouse keratometer. The resulting topography and pachymetry maps are analogous to those used diagnostically in clinic and potentially allow for characterization of genetically modified mice that replicate key features of human corneal disease.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

MeSH terms

Animals
Biometry
Cornea / anatomy & histology*
Corneal Pachymetry*
Corneal Topography*
Male
Mice
Mice, Inbred C57BL
Models, Theoretical
Tomography, Optical Coherence / methods*

Abstract

Publication types

MeSH terms

Grants and funding