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Tissue Eng Part A. 2019 Nov 7. doi: 10.1089/ten.TEA.2019.0188. [Epub ahead of print]

Investigation on ciliary functionality of different airway epithelial cell lines in 3D cell culture.

Author information

1
University Hospital Würzburg, Chair of Tissue Engineering and Regenerative Medicine, Würzburg, Germany.
2
University Hospital Würzburg, Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Würzburg, Deutschland, Germany; nina.lodes@uni-wuerzburg.de.
3
University Hospital Würzburg, Chair of Tissue Engineering and Regenerative Medicine, Würzburg, Germany; K.Seidensticker@gmx.de.
4
Justus Liebig University Giessen Faculty of Medicine, 60633, Institute for Anatomy and Cell Biology, Giessen, Hessen, Germany; Alexander.perniss@anatomie.med.uni-giessen.de.
5
University Hospital Wuerzburg, Tissue Engineering and Regenerative Medicine, Röntgenring 11, Würzburg, Bavaria, Germany, 97070; Sarah.nietzer@uni-wuerzburg.de.
6
University Hospital Würzburg, Chair of Tissue Engineering and Regenerative Medicine, Würzburg, Germany; heike.oberwinkler@uni-wuerzburg.de.
7
Inscreenex, Braunschweig, Germany; tobias.may@inscreenex.com.
8
University Hospital Magdeburg, 39067, Deptartment of Thoracic Surgery, Magdeburg, Sachsen-Anhalt, Germany; thorsten.walles@med.ovgu.de.
9
University Hospital Würzburg, Department of Pediatrics, Würzburg, Germany; hebestreit_h@ukw.de.
10
University Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Universitiy Hospital Wuerzburg, Wuerzburg, Germany; hackenberg_s@ukw.de.
11
Fraunhofer ISC, Röntgenring 11, Würzburg, Germany, 97070.
12
University Hospital Würzburg, Chair of Tissue Engineering and Regenerative Medicine, Röntgenring 11, Würzburg, Germany, 97070; maria.steinke@isc.fraunhofer.de.

Abstract

3D respiratory tissue models have been generated using, for example, human primary airway epithelial cells (hAEC) or respective cell lines. To investigate ciliopathies, such as primary ciliary dyskinesia, the presence of functional kinocilia in vitro is an essential prerequisite. Since access to hAEC of healthy donors is limited, we aimed to identify a respiratory epithelial cell line that is capable to display functional kinocilia on at least 60 % of the apical surface. Thus, we cultured four different human respiratory cell lines with human primary airway fibroblasts under airlift conditions, characterized the morphology and analyzed ciliary function. Only one of the tested cell lines showed beating kinocilia, however, less than 10 % of the whole surface was covered and ciliary beating was undirected. Positive control tissue models using hAEC and fibroblasts displayed expected directed ciliary beating pattern around 11 Hz. Our data show that the available cell lines are not suitable for basic and applied research questions whenever functional kinocilia are required and that, rather, hAEC- or human induced pluripotent stem cells-derived tissue models need to be generated.

PMID:
31696788
DOI:
10.1089/ten.TEA.2019.0188

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