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Nanomedicine (Lond). 2019 Dec;14(23):3013-3033. doi: 10.2217/nnm-2019-0206. Epub 2019 Nov 7.

Design of polymeric nanocapsules to improve their lympho-targeting capacity.

Author information

1
Center for Research in Molecular Medicine & Chronic Diseases (CIMUS), Health Research Institute of Santiago de Compostela (IDIS), School of Pharmacy, Universidade de Santiago de Compostela, Campus Vida, 15706 Santiago de Compostela, Spain.
2
Institute for Research in Biomedicine, Università della Svizzera Italiana, via Vincenzo Vela 6, 6500 Bellinzona, Switzerland.
3
Graduate School of Cellular and Biomedical Sciences, Faculty of Medicine, University of Bern, 3012 Bern, Switzerland.
4
Department of Biochemistry & Molecular Biology, School of Pharmacy, Universidade de Santiago de Compostela, Campus Vida s/n, 15782 Santiago, A Coruña, Spain.

Abstract

Aim: To design lympho-targeted nanocarriers with the capacity to enhance the activity of associated drugs/antigens whose target is within the lymphatic system. Materials & methods: Inulin (INU)-based nanocapsules (NCs), negatively charged and positively charged chitosan NCs were prepared by the solvent displacement techniques. The NCs were produced in two sizes: small (70 nm) and medium (170-250 nm). Results: In vitro results indicated that small NCs interacted more efficiently with dendritic cells than the larger ones. The study of the NCs biodistribution in mice, using 3D reconstruction of the popliteal lymph node, showed that small INU NCs have the greatest access and uniform accumulation in different subsets of resident immune cells. Conclusion: Small and negatively charged INU NCs have a potential as lympho-targeted antigen/drug nanocarriers.

KEYWORDS:

chitosan; dendritic cells; inulin; lymph nodes; nanocapsules; particle size; targeting; two-photon microscopy

PMID:
31696773
DOI:
10.2217/nnm-2019-0206

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