Format

Send to

Choose Destination
J Mass Spectrom. 2019 Nov 6. doi: 10.1002/jms.4458. [Epub ahead of print]

Optimization of a minimal sample preparation protocol for imaging mass spectrometry of unsectioned juvenile invertebrates.

Author information

1
Department of Pharmaceutical Sciences, University of Illinois at Chicago, 833 S Wood St, Chicago, IL, 60612, USA.
2
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI, 53706, USA.
3
Department of Microbiology - Immunology, Northwestern University Feinberg School of Medicine, 320 E Superior Street, Chicago, IL, 60611, USA.

Abstract

Tissue sections have long been the subject matter for the application of imaging mass spectrometry, but recently the technique has been adapted for many other purposes including bacterial colonies and 3D cell culture. Here, we present a simple preparation method for unsectioned invertebrate tissue without the need for fixing, embedding, or slicing. The protocol was used to successfully prepare a Hawaiian bobtail squid hatchling for analysis, and the resulting data detected ions that correspond to compounds present in the host only during its symbiotic colonization by Vibrio fischeri.

KEYWORDS:

Euprymna scolopes; microbiology; symbiosis; unsectioned organism imaging

PMID:
31693273
DOI:
10.1002/jms.4458

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center