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Biochem Cell Biol. 2019 Nov 5. doi: 10.1139/bcb-2019-0156. [Epub ahead of print]

Nicotine-upregulated miR-30a arrests cell cycle in G1 phase by directly targeting CCNE2 in human periodontal ligament cells.

Author information

1
Characteristic Medical Center of the Chinese people's Armed Police Force, Department of Stomatology, Tianjin, China.
2
Fourth Military Medical University, 12644, School of Stomatology, State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, Xi'an, Shaanxi, China ; pediatricwu@163.com.
3
College of Stomatology, Xi'an Jiaotong University, Department of Orthodontics, Xi'an, China ; 941139233@qq.com.
4
Characteristic Medical Center of the Chinese people's Armed Police Force, Department of Stomatology, Tianjin, China ; 77231038@qq.com.
5
Fourth Military Medical University, 12644, School of Stomatology, State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, Xi'an, Shaanxi, China ; wxjingpd@hotmail.com.

Abstract

Both tobacco smoking and nicotine have been reported to regulate the occurrence and progression of periodontitis. Many studies have demonstrated that nicotine destroys regeneration of periodontal tissues primarily by inhibiting the proliferation of human periodontal ligament (PDL) cells. However, the mechanism underlying this process is still unclear. Therefore, we investigated whether nicotine-upregulated miR-30a inhibited the proliferation of human PDL cells by downregulating cyclin E2 (CCNE2) in vitro. Quantitative real-time PCR analysis revealed that nicotine upregulated the expression of miR-30a in human PDL cells. In addition, nicotine could inhibit the proliferation of human PDL cells by inducing cell cycle arrest. To support this hypothesis, we showed that nicotine downregulated the expression of CCNE2 in human PDL cells, whereas inhibition of miR-30a restored CCNE2 expression that were downregulated by nicotine. Furthermore, we found that miR-30a directly interacts with the CCNE2 3'UTR through luciferase reporter assay. In conclusion, these findings indicate that nicotine-upregulated miR-30a inhibits the proliferation of human PDL cells by downregulating the expression of CCNE2.

PMID:
31689122
DOI:
10.1139/bcb-2019-0156

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