Format

Send to

Choose Destination
Onco Targets Ther. 2019 Sep 13;12:7551-7560. doi: 10.2147/OTT.S212649. eCollection 2019.

Restoration of miR-26b expression partially reverses the cisplatin resistance of NSCLC by targeting tafazzin.

Author information

1
Respiratory Ward 1, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, People's Republic of China.
2
Respiratory Intensive Care Unit, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, People's Republic of China.
3
Respiratory Ward 2, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, People's Republic of China.
4
Clinical Pharmacy, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, People's Republic of China.

Abstract

Background:

Dysregulation of microRNAs has been reported to be responsible for drug resistance of cancers. However, the association between aberrant expression of miR-26b and cisplatin resistance in non-small cell lung cancer (NSCLC) remains unclear.

Methods:

PC9 and A549 were used to establish the cisplatin resistance models on NSCLC. Expression of miR-26b in cisplatin-resistant PC9 and A549 cells (PC9/R and A549/R) was detected by quantitative real-time PCR assays. Drug sensitivity and mitochondrial apoptosis were detected by Cell Counting Kit-8 assay and flow cytometry assay, respectively. The target relationship between miR-26b and tafazzin (TAZ) was validated by dual-luciferase reporter assay.

Results:

Obvious downregulation of miR-26b was observed in PC9/R and A549/R cells. Restoration of miR-26b partially reversed the cisplatin resistance of PC9/R and A549/R cells. Expression of TAZ was increased in PC9/R and A549/R cells compared to the parental PC9 and A549 cells. Results of dual-luciferase reporter assays verified that TAZ was targeted by miR-26b. We showed that restoration of miR-26b expression inhibited the TAZ expression and thus expanded the mitochondrial pathway of apoptosis induced by cisplatin in PC9/R and A549/R cells.

Conclusion:

Restoration of miR-26b expression partially reverses the cisplatin resistance of NSCLC by targeting TAZ. miR-26b/TAZ axis may represent a potential strategy to reverse the cisplatin in NSCLC.

KEYWORDS:

NSCLC; TAZ; cisplatin; miR-26b; resistance

Supplemental Content

Full text links

Icon for Dove Medical Press Icon for PubMed Central
Loading ...
Support Center