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Oral Maxillofac Surg. 2019 Dec;23(4):473-479. doi: 10.1007/s10006-019-00807-9. Epub 2019 Nov 4.

Imaging and histomorphometric evaluation of mandible and tibia of rats treated with bisphosphonates.

Author information

1
Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
2
Graduate Program in Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
3
Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, RS, Brazil. carolinauv@gmail.com.
4
Serviço de Patologia Bucal, Faculdade de Odontologia, Universidade Federal de Pelotas, Rua Gonçalves Chaves, 457, Sala 607, Pelotas, RS, CEP: 96015-560, Brazil. carolinauv@gmail.com.

Abstract

OBJECTIVES:

To evaluate the mandible and tibia of rats treated with bisphosphonates (BPs) by imaging and histomorphometric analysis.

STUDY DESIGN:

Thirty-four rat specimens (Rattus norvegicus, Wistar strain) were distributed into 3 groups: (1) 12 rats treated with zoledronic acid; (2) 12 rats treated with clodronate; and (3) the control group, containing 10 rats that received saline. All bones were exposed to cone beam computed tomography (CBCT). The images were analyzed to determine bone density (BD), using the software OsiriX 7.0. Histological slides were prepared from the specimens and the proportion of bone volume (BV) was quantified using the software Adobe Photoshop CC.

RESULTS:

There was no statistically significant difference in BD either between the drug groups or between mandible and tibia. BV between BPs and control group did not show a significant difference. However, comparing the two bones, the mandibles in the control group displayed higher BV than did the tibiae in the same group.

CONCLUSION:

According to our results, we conclude that (1) BD was not altered by bone type or by type of BP administered, and (2) treatment with zoledronic acid or clodronate did not affect BV in the mandible or tibia of test groups.

KEYWORDS:

Bisphosphonate; Bisphosphonate-associated osteonecrosis of the jaw; Bone tissue; Cone-beam computed tomography; Jaws

PMID:
31686251
DOI:
10.1007/s10006-019-00807-9

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