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J Clin Endocrinol Metab. 2019 Nov 4. pii: dgz176. doi: 10.1210/clinem/dgz176. [Epub ahead of print]

Shotgun lipidomics discovered diurnal regulation of lipid metabolism linked to insulin sensitivity in non-diabetic men.

Kessler K1,2,3,4, Gerl MJ5, Hornemann S1,2, Damm M5, Klose C5, Petzke KJ6, Kemper M1,2,3, Weber D7,8, Rudovich N1,2,3,9, Grune T2,7,8,10,11, Simons K5,12, Kramer A13, Pfeiffer AFH1,2,3, Pivovarova-Ramich O1,2,3,14.

Author information

1
Dept. of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
2
German Center for Diabetes Research (DZD), München-Neuherberg, Germany.
3
Dept. of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University of Medicine, Berlin, Germany.
4
Biomineral Research Group, Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
5
Lipotype GmbH, Tatzberg, Dresden, Germany.
6
Research Group Physiology of Energy Metabolism, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
7
Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
8
NutriAct-Competence Cluster Nutrition Research Berlin-Potsdam, Nuthetal, Germany.
9
Division of Endocrinology and Diabetes, Department of Internal Medicine, Spital Bülach, Bülach, Switzerland.
10
German Center for Cardiovascular Research (DZHK), Berlin, Germany.
11
Institute of Nutrition, University of Potsdam, Nuthetal, Germany.
12
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
13
Laboratory of Chronobiology, Institute for Medical Immunology, Charité University of Medicine, Berlin, Germany.
14
Reseach Group Molecular Nutritional Medicine, Dept. of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.

Abstract

CONTEXT:

Meal timing affects metabolic homeostasis and body weight, but how composition and timing of meals affect plasma lipidomics in humans is not well studied.

OBJECTIVE:

We used high throughput shotgun plasma lipidomics to investigate effects of timing of carbohydrate and fat intake on lipid metabolism and its relation to glycaemic control.

DESIGN:

29 non-diabetic men consumed (i) a high-carb test meal (MTT-HC) at 09:00 and a high-fat meal (MTT-HF) at 15:40; or (ii) MTT-HF at 09:00 and MTT-HC at 15:40. Blood was sampled before and 180 min after completion of each MTT. Subcutaneous adipose tissue (SAT) was collected after overnight fast and both MTTs. Prior to each investigation day, participants consumed a 4-week isocaloric diet of the same composition: (1) high-carb meals until 13:30 and high-fat meals between 16:30 and 22:00 or (2) the inverse order.

RESULTS:

12h-daily lipid patterns showed a complex regulation by both the time of day (67.8%) and meal composition (55.4%). A third of lipids showed a diurnal variation in postprandial responses to the same meal with mostly higher responses in the morning than in the afternoon. Triacylglycerols containing shorter and more saturated fatty acids were enriched in the morning. SAT transcripts involved in fatty acid synthesis and desaturation showed no diurnal variation. Diurnal changes of seven lipid classes were negatively associated with insulin sensitivity, but not with glucose and insulin response or insulin secretion.

CONCLUSIONS:

This study identified postprandial plasma lipid profiles as being strongly affected by meal timing and associated with insulin sensitivity.

KEYWORDS:

circadian clock; glucose metabolism; insulin sensitivity; lipid metabolism; meal timing; plasma lipidome

PMID:
31680138
DOI:
10.1210/clinem/dgz176

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