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Pigment Cell Melanoma Res. 2019 Nov 2. doi: 10.1111/pcmr.12842. [Epub ahead of print]

Sequencing two Tyr::CreERT2 transgenic mouse lines.

Aktary Z1,2,3, Corvelo A4, Estrin C1,2,3, Larue L1,2,3.

Author information

1
Institut Curie, PSL Research University, INSERM U1021, Normal and Pathological Development of Melanocytes, Orsay, France.
2
Univ Paris-Sud, Univ Paris-Saclay, CNRS, UMR 3347, Orsay, France.
3
Equipes Labellisées Ligue Contre le Cancer.
4
New York Genome Center, New York, USA.

Abstract

The Cre/loxP system is a powerful tool that has allowed the study of the effects of specific genes of interest in various biological settings. The Tyr::CreERT2 system allows for the targeted expression and activity of the Cre enzyme in the melanocyte lineage following treatment with tamoxifen, thus providing spatial and temporal control of the expression of specific target genes. Two independent transgenic mouse models, each containing a Tyr::CreERT2 transgene, have been generated and are widely used to study melanocyte transformation. In this study, we performed whole genome sequencing (WGS) on genomic DNA from the two Tyr::CreERT2 mouse models and identified their sites of integration in the C57BL/6 genome. Based on these results, we designed PCR primers to accurately, and efficiently, genotype transgenic mice. Finally, we discussed some of the advantages of each transgenic mouse model.

KEYWORDS:

Nlrp12; Suv39h2; Tyr::CreERT2; Whole genome sequencing; chromosome 2; chromosome 7; melanocyte; melanoma; miR-290-295

PMID:
31679174
DOI:
10.1111/pcmr.12842

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