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Protein Expr Purif. 2019 Oct 31;167:105524. doi: 10.1016/j.pep.2019.105524. [Epub ahead of print]

Expression and purification of recombinant G protein-coupled receptors: A review.

Author information

1
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: d.wiseman1@aston.ac.uk.
2
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: otcherea@aston.ac.uk.
3
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: patelj47@aston.ac.uk.
4
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: uddinr3@aston.ac.uk.
5
University of Birmingham, Birmingham, B15 2TT, UK. Electronic address: n.pollock@bham.ac.uk.
6
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: s.routledge@aston.ac.uk.
7
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: a.rothnie@aston.ac.uk.
8
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: c.slack@aston.ac.uk.
9
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: d.r.poyner@aston.ac.uk.
10
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: r.m.bill@aston.ac.uk.
11
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address: a.goddard@aston.ac.uk.

Abstract

Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.

KEYWORDS:

Expression; GPCR; Purification; Review; SMALP

PMID:
31678667
DOI:
10.1016/j.pep.2019.105524
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