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Biomed Pharmacother. 2019 Oct 25;120:109567. doi: 10.1016/j.biopha.2019.109567. [Epub ahead of print]

Icariin modulates carrageenan-induced acute inflammation through HO-1/Nrf2 and NF-kB signaling pathways.

Author information

1
Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, Egypt. Electronic address: nagla_fouad@yahoo.com.
2
Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Cellular inflammation has been recognized as the leading factor in numerous diseases, causing either cell death, organ-specific damage, or genesis of different cancers. Icariin, a flavonol glucoside, extracted from Herba Epimedii, offers various pharmacological activities, including reducing inflammation and an antioxidant effect. We aimed to evaluate the anti-inflammatory mechanisms of icariin focussing on both HO-1/Nrf2 and NF-kB pathways. Acute inflammation was induced in rats via carrageenan in this study. Icariin was injected in a dose of 50 mg/kg. Icariin significantly reduced paw swelling in carrageenan-injected animals. It also ameliorated carrageenan-induced paw histopathological alterations. Icariin significantly increased paw enzymatic and non-enzymatic antioxidants. It decreased paw lipid peroxidation. Icariin decreased paw levels of inflammatory cytokines and NF-kB. The immunohistochemical analysis showed that icariin significantly increased Nrf2 gene expression while decreasing NF-kB and COX-2 gene expression. RT-PCR analysis revealed that icariin injection significantly increased both Nrf2 and HO-1 expression of mRNA. The results of this study collectively show that icariin improved carrageenan-induced paw edema by modulating HO-1/Nrf2 and NF-ĸB signaling.

KEYWORDS:

Carrageenan; HO-1; Icariin; Inflammatory cytokines; NF-kB; Nrf2

PMID:
31670031
DOI:
10.1016/j.biopha.2019.109567
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