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Enferm Infecc Microbiol Clin. 2019 Oct 24. pii: S0213-005X(19)30231-9. doi: 10.1016/j.eimc.2019.08.006. [Epub ahead of print]

Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children.

[Article in English, Spanish]

Author information

1
Instituto de Medicina Tropical "Alexander von Humboldt", Universidad Peruana Cayetano Heredia, Lima, Peru.
2
Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
3
ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
4
Instituto de Medicina Tropical "Alexander von Humboldt", Universidad Peruana Cayetano Heredia, Lima, Peru; University of Texas Health Science Center at Houston, School of Public Health, Houston, USA. Electronic address: Theresa.J.Ochoa@uth.tmc.edu.

Abstract

INTRODUCTION:

We evaluated the presence of sIgA in saliva, versus Escherichia coli secreted proteins (Esp) related to the type III secretion system (T3SS), and its semi-quantitative concentration in children under 2 years-old (no longer breastfed) who were previously colonized or infected with enteropathogenic E. coli (EPEC).

METHODS:

We analyzed the presence of sIgA in 40 children, who previously had positive cultures for EPEC associated (n=17) or not associated (n=23) with diarrhea, using the Western Blot technique versus E. coli secreted proteins: EspABCD. A semi-quantitative measurement of the reaction for each protein was made by its density peaks (OD).

RESULTS:

We found sIgA versus all or some EspABCD proteins in both groups. However, the ill patients had higher concentrations of these antibodies than colonized patients.

DISCUSSION:

The presence of sIgA in saliva could reflect an intestinal immune response and their levels could be related to a greater exposure and/or bacterial load.

KEYWORDS:

Children; Inmunoglobulina A secretoria en saliva; Niños; Proteínas de virulencia; Secretory immunoglobulin A in saliva; Sistema de secreción tipo III; Type III secretion system; Virulence proteins

PMID:
31668862
DOI:
10.1016/j.eimc.2019.08.006

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