Format

Send to

Choose Destination
Lab Anim. 2019 Oct 29:23677219883327. doi: 10.1177/0023677219883327. [Epub ahead of print]

Performance of severity parameters to detect chemotherapy-induced pain and distress in mice.

Author information

1
Institute for Experimental Molecular Imaging, Medical Faculty, RWTH Aachen International University, Germany.
2
Department of Nuclear Medicine, Medical Faculty, RWTH Aachen International University, Germany.
3
Department of Radiology and Nuclear Medicine, Maastricht University Medical Center (MUMC+), The Netherlands.
4
Institute for Laboratory Animal Science, Medical Faculty, RWTH Aachen International University, Germany.

Abstract

According to European Union directive 2010/63/EU a severity classification of experimental procedures performed on laboratory animals is mandatory. This includes a prospective evaluation of all interventions performed within the experiment, as well as an assessment of the actual burden of each animal during the experiment. In this regard, the evaluation and scoring of defined criteria regarding the health state of animals could help to early identify deteriorations in animal health and facilitate the application of humane endpoints. This article discusses the applicability of an adapted score sheet in BALB/cAnNRj mice receiving either cisplatin, doxorubicin or busulfan, three chemotherapeutic agents with different toxicological profiles and longitudinal non-invasive molecular imaging. The health state was investigated by score sheets documenting general state, body weight, spontaneous behaviour and treatment specific parameters (e.g. anaemia, neurotoxicity, persistent diarrhoea). Although blood and serum analyses clearly indicated various organ damage, most scoring parameters except for body weight did not report on the deceasing animal health state. Thus, there is need for more sensitive observational parameters to judge the animal's health state and welfare.

KEYWORDS:

Severity assessment; chemotherapy; imaging; score sheet

PMID:
31660776
DOI:
10.1177/0023677219883327

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center