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Sci Rep. 2019 Oct 28;9(1):15385. doi: 10.1038/s41598-019-51673-0.

Comparative analysis of obesity-related cardiometabolic and renal biomarkers in human plasma and serum.

Author information

1
Department of Molecular and Clinical Medicine, Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden.
2
Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden.
3
Department of Cell & Molecular Biology, Uppsala University, Uppsala, Sweden.
4
NIH West Coast Metabolomics Center, Genome Center, University of California Davis, Davis, USA.
5
Department of Psychiatry and Neurochemistry, Institute of Physiology and Neuroscience, University of Gothenburg, Gothenburg, Sweden.
6
King's College London, Institute of Psychiatry, Psychology & Neuroscience, Maurice Wohl Clinical Neuroscience Institute, London, UK.
7
NIHR Biomedical Research Centre for Mental Health & Biomedical Research Unit for Dementia at South London & Maudsley NHS Foundation, London, UK.
8
Department of Gastrosurgical Research and Education, Institute of Clinical Sciences, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden.
9
Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
10
Cardiovascular Research Centre, School of Medical Sciences, Örebro University, Örebro, Sweden.
11
Dementia Research Centre, Institute of Neurology, University College London, London, UK.
12
Department of Nutrition, University of California Davis, Davis, USA.
13
USDA, ARS, Western Human Nutrition Research Center, Davis, USA.
14
Division of Cardiology, School of Medicine, University of California San Diego, San Diego, USA.
15
Department of Molecular and Clinical Medicine, Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden. emma.borgeson@wlab.gu.se.
16
Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden. emma.borgeson@wlab.gu.se.
17
Department of Clinical Physiology, Sahlgrenska University Hospital, Gothenburg, Sweden. emma.borgeson@wlab.gu.se.

Abstract

The search for biomarkers associated with obesity-related diseases is ongoing, but it is not clear whether plasma and serum can be used interchangeably in this process. Here we used high-throughput screening to analyze 358 proteins and 76 lipids, selected because of their relevance to obesity-associated diseases, in plasma and serum from age- and sex-matched lean and obese humans. Most of the proteins/lipids had similar concentrations in plasma and serum, but a subset showed significant differences. Notably, a key marker of cardiovascular disease PAI-1 showed a difference in concentration between the obese and lean groups only in plasma. Furthermore, some biomarkers showed poor correlations between plasma and serum, including PCSK9, an important regulator of cholesterol homeostasis. Collectively, our results show that the choice of biofluid may impact study outcome when screening for obesity-related biomarkers and we identify several markers where this will be the case.

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