Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)

Angew Chem Int Ed Engl. 2020 Feb 3;59(6):2380-2384. doi: 10.1002/anie.201912211. Epub 2019 Dec 19.

Abstract

Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.

Keywords: NMR spectroscopy; automation; projection spectroscopy; proton detection; solid-state structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation
  • Isotope Labeling
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Proteins / chemistry*
  • Superoxide Dismutase / chemistry
  • beta 2-Microglobulin / chemistry

Substances

  • Proteins
  • beta 2-Microglobulin
  • Superoxide Dismutase