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Nutrients. 2019 Oct 23;11(11). pii: E2554. doi: 10.3390/nu11112554.

Docosahexaenoic Acid Inhibits PTP1B Phosphatase and the Viability of MCF-7 Breast Cancer Cells.

Author information

1
Department of Medical Chemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland. alicjakuban@gumed.edu.pl.
2
Department of Medical Chemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland. m.gorska@gumed.edu.pl.
3
Institute of Biomaterials and Biomolecular Systems, Department of Biophysics, University of Stuttgart, 70550 Stuttgart, Germany. m.gorska@gumed.edu.pl.
4
The Euro-Mediterranean Institute of Science and Technology, 90127 Palermo, Italy. m.gorska@gumed.edu.pl.
5
Li Ka Shing Applied Virology Institute, Department of Medical Microbiology and Immunology 6-020 Katz Group Centre, University of Alberta, Edmonton, AB T6G 2E1, Canada. ksahu@ualberta.ca.
6
Department of Medical Chemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland. tomasz.kostrzewa@gumed.edu.pl.
7
Department of Medical Chemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland. mwozniak@gumed.edu.pl.
8
Department of Oncology, University of Alberta, Edmonton, AB T6G 1Z2, Canada. jack.tuszynski@gmail.com.
9
Department of Physics, CCIS, University of Alberta, Edmonton, AB T6G 2E1, Canada. jack.tuszynski@gmail.com.
10
DIMEAS, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy. jack.tuszynski@gmail.com.

Abstract

BACKGROUND:

Docosahexaenoic acid (DHA) is an essential polyunsaturated fatty acid compound present in deep water fishes and dietary supplements, with a wide spectrum of potential health benefits, ranging from neurological to anti-inflammatory.

METHODS:

Due to the fact that DHA is considered a breast cancer risk reducer, we examined the impact of DHA on MCF-7 breast cancer cells' viability and its inhibitory properties on protein tyrosine phosphatase 1B (PTP1B), a pro-oncogenic phosphatase.

RESULTS:

We found that DHA is able to lower both the enzymatic activity of PTP1B phosphatase and the viability of MCF-7 breast cancer cells. We showed that unsaturated DHA possesses a significantly higher inhibitory activity toward PTP1B in comparison to similar fatty acids. We also performed a computational analysis of DHA binding to PTP1B and discovered that it is able to bind to an allosteric binding site.

CONCLUSIONS:

Utilizing both a recombinant enzyme and cellular models, we demonstrated that DHA can be considered a potential pharmacological agent for the prevention of breast cancer.

KEYWORDS:

breast cancer; docosahexaenoic acid; omega-3 acids; protein tyrosine phosphatase PTP1B

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