Format

Send to

Choose Destination
Viruses. 2019 Oct 22;11(10). pii: E971. doi: 10.3390/v11100971.

High Diversity and Novel Enteric Viruses in Fecal Viromes of Healthy Wild and Captive Thai Cynomolgus Macaques (Macaca fascicularis).

Author information

1
Vitalant Research Institute, San Francisco, CA 94118, USA. vorthon007.giftedcru@gmail.com.
2
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. vorthon007.giftedcru@gmail.com.
3
Vitalant Research Institute, San Francisco, CA 94118, USA. efahsbender@vitalant.org.
4
Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA 9413, USA. efahsbender@vitalant.org.
5
Vitalant Research Institute, San Francisco, CA 94118, USA. EAltan@vitalant.org.
6
Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA 9413, USA. EAltan@vitalant.org.
7
National Primate Research Center-Chulalongkorn University, Saraburi 18110, Thailand. taratorn.kem@gmail.com.
8
Vitalant Research Institute, San Francisco, CA 94118, USA. xdeng@vitalant.org.
9
National Primate Research Center-Chulalongkorn University, Saraburi 18110, Thailand. suchinda.m@chula.ac.th.
10
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. sp.medbiochemcu@gmail.com.
11
Center of Excellence in Systems Biology, Chulalongkorn University (CUSB), Bangkok 10330, Thailand. sp.medbiochemcu@gmail.com.
12
Vitalant Research Institute, San Francisco, CA 94118, USA. Eric.Delwart@ucsf.edu.
13
Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA 9413, USA. Eric.Delwart@ucsf.edu.

Abstract

Cynomolgus macaques are common across South East Asian countries including Thailand. The National Primate Research Center of Thailand, Chulalongkorn University (NPRCT-CU) captures wild-borne cynomolgus macaque for research use. Limited information is available on the enteric viruses and possible zoonotic infections into or from cynomolgus macaques. We characterized and compare the fecal virome of two populations; healthy wild-originated captive cynomolgus macaques (n = 43) reared in NPRCT-CU and healthy wild cynomolgus macaques (n = 35). Over 90% of recognized viral sequence reads amplified from feces were from bacterial viruses. Viruses from seven families of mammalian viruses were also detected (Parvoviridae, Anelloviridae, Picornaviridae, Adenoviridae, Papillomaviridae, Herpesviridae, and Caliciviridae). The genomes of a member of a new picornavirus genus we named Mafapivirus, a primate chapparvovirus, and a circular Rep-encoding single-strand (CRESS) DNA virus were also characterized. Higher abundance of CRESS DNA viruses of unknown tropism and invertebrate-tropic ambidensovirus were detected in wild versus captive macaques likely reflecting dietary differences. Short term rearing in captivity did not have a pronounced effect on the diversity of mammalian viruses of wild cynomolgus macaques. This study is the first report of the fecal virome of cynomolgus macaques, non-human primates frequently used in biomedical research and vaccination studies.

KEYWORDS:

high-throughput sequencing; long-tailed macaque; viral discovery; virome

PMID:
31652508
DOI:
10.3390/v11100971
Free full text

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI)
Loading ...
Support Center