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BMC Vet Res. 2019 Oct 22;15(1):359. doi: 10.1186/s12917-019-2114-3.

Evaluation of Mycobacterium tuberculosis lipoarabinomannan antigen assay and rapid serology blood test for the diagnosis of bovine tuberculosis in Ethiopia.

Author information

1
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
2
Nationwide Children's Hospital, Section of Infectious Diseases, College of Medicine, The Ohio State University, Columbus, OH, USA.
3
Center for Biostatistics, College of Medicine, The Ohio State University, Columbus, OH, USA.
4
Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH, USA.
5
Global One Health initiative (GOHi), The Ohio State University, Columbus, OH, USA.
6
Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
7
Department of Microbial Infection and Immunity, College of Medicine, The Ohio State University, Columbus, OH, USA.
8
Present Address: Texas Biomedical Research Institute, San Antonio, TX, USA.
9
Global One Health initiative (GOHi), The Ohio State University, Columbus, OH, USA. Shu-Hua.Wang@osumc.edu.
10
Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, OH, USA. Shu-Hua.Wang@osumc.edu.

Abstract

BACKGROUND:

Bovine tuberculosis (bTB) is prevalent in dairy cattle in Ethiopia. Currently used diagnostic tools such as the single intradermal comparative tuberculin test (SICTT) are time consuming and labor intensive. A rapid, easy-to-use and cost-effective diagnostic test would greatly contribute to the control of bTB in developing countries like Ethiopia. In the present study, two point-of-care diagnostic tests were evaluated for the detection of bTB: LIONEX® Animal TB Rapid test, a membrane-based test for the detection of antibodies to Mycobacterium bovis in blood and ALERE® Determine TB Lipoarabinomannan (LAM) Ag, an immunoassay for the detection of lipoarabinomannan (LAM) antigen (Ag) of mycobacteria in urine. A combination of the SICTT and gamma interferon (IFN-γ) test was used as the gold standard for the validation of these point-of-care tests, as it was not feasible to slaughter the study animals to carry out the historical gold standard of mycobacterial culture. A total of 175 heads of cattle having three different bTB infection categories (positive SICTT, negative SICTT, and unknown SICTT status) were used for this study.

RESULT:

The sensitivity and specificity of TB LAM Ag were 72.2% (95% CI = 62.2, 80.4) and 98.8% (95% CI = 93.6, 99.7), respectively, while the sensitivity and specificity of the LIONEX Animal TB rapid test assay were 54% (95% CI = 44.1 64.3) and 98.8% (95% CI = 93.6, 99.7) respectively. The agreement between TB LAM Ag and SICTT was higher (κ = 0.85; 95% CI = 0.65-0.94) than between TB LAM Ag and IFN-γ (κ = 0.67; 95% CI = 0.52-0.81). The agreement between LIONEX Animals TB Rapid blood test and SICTT was substantial, (κ = 0.63; 95% CI = 0.49-0.77) while the agreement between LIONEX Animal TB rapid blood test and IFN-γ test was moderate (κ = 0.53; 95% CI = 0.40-0.67). Analysis of receiver operating curve (ROC) indicated that the area under the ROC curve (AUC) for TB LAM Ag was 0.85 (95% CI = 0.79-0.91) while it was 0.76 (95% CI; =0.69-0.83) for LIONEX Animal TB rapid test assay.

CONCLUSION:

This study showed that TB LAM Ag had a better diagnostic performance and could potentially be used as ancillary either to SICTT or IFN-γ test for diagnosis of bTB.

KEYWORDS:

Bovine tuberculosis; Diagnostic performance; Ethiopia; LIONEX animal TB rapid; TB LAM antigen

PMID:
31640672
PMCID:
PMC6805383
DOI:
10.1186/s12917-019-2114-3
[Indexed for MEDLINE]
Free PMC Article

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