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Biomed Chromatogr. 2019 Oct 21:e4717. doi: 10.1002/bmc.4717. [Epub ahead of print]

Validation and application of a novel UHPLC-MS/MS method for the measurement of furanodienone in rat plasma.

Author information

1
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, China.
2
Shandong Medical College, Jinan, China.
3
Jiaozhou People's Hospital, Qingdao, China.

Abstract

A sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established to analyze furanodienone in rat plasma. In the process of chromatographic separation, selected reaction monitoring transitions for furanodienone and patchouli alcohol (internal standard, IS) were m/z 231.1 → 83.2 and m/z 205.1 → 95.1, respectively. Great linearity of furanodienone in plasma samples was found in the corresponding concentration range (r > 0.995). Intra- and inter-day precisions (RSD, %) were <11.3% in plasma, and the accuracy (RE, %) was within ±10.7%. This method was used to the furanodienone study on rat pharmacokinetics after a single oral dose of 10 mg/kg of furanodiene. The results indicated that the maximum observed plasma concentration was 52.4 ± 19.1 ng/ml at 1.2 ± 0.7 h with an elimination half-life of 2.2 ± 0.7 h. The obtained data indicated that furanodienone could be moderately distributed and eliminated.

KEYWORDS:

UHPLC-MS/MS; diene-type sesquiterpene; furanodienone; pharmacokinetics

PMID:
31634986
DOI:
10.1002/bmc.4717

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