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Dev Comp Immunol. 2020 Mar;104:103524. doi: 10.1016/j.dci.2019.103524. Epub 2019 Oct 18.

Spatial and temporal transcriptomic analyses reveal locust initiation of immune responses to Metarhizium acridum at the pre-penetration stage.

Author information

1
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Bldg. 981, Museum Rd, Gainesville, FL32611, USA. Electronic address: zhangwei976@cqu.edu.cn.
2
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China; College of Preclinical Medicine, Southwest Medical University, Luzhou, 646000, PR China. Electronic address: xlzheng1111@126.com.
3
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China. Electronic address: chenjianhong777@163.com.
4
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Bldg. 981, Museum Rd, Gainesville, FL32611, USA. Electronic address: keyhani@ufl.edu.
5
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China. Electronic address: kaiyong_cai@cqu.edu.cn.
6
School of Life Sciences, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China. Electronic address: yuxianxia@cqu.edu.cn.

Abstract

Insect hemocyte and fat body tissues play critical functional roles in insect immunity. Little, however, is known concerning the dynamic responses of these tissues to fungal infection. Here, we report on a time course of locust hemocyte and fat body transcriptomic responses to infection by the acridid specific fungal pathogen, Metarhizium acridum. Fat body responses were more pronounced at all infection stages as compared to hemocytes. Immune and other related genes were induced far earlier than previously considered including at pre-penetration stages. Differential expression in hemocyte and fat body tissues persisted throughout the course of infection up until host death. Our data indicate selective pressure on the host to recognize the infection as early as possible in order to limit its spread. Overall, fat body and hemocyte tissues launch a robust multi-tiered response to combat the fungal pathogen, with our data providing potential host targets for exploitation in pest control.

KEYWORDS:

Fat body; Hemocytes; Infection time course; Locust; Metarhizium; Transcriptomics

PMID:
31634520
DOI:
10.1016/j.dci.2019.103524

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