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PLoS Biol. 2019 Oct 21;17(10):e3000081. doi: 10.1371/journal.pbio.3000081. eCollection 2019 Oct.

High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation-associated versus preneurulation-associated fate patterning.

Author information

1
Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada.
2
Collaborative Program in Developmental Biology, University of Toronto, Toronto, Ontario, Canada.
3
Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
4
Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom.
5
Research Group Reproduction and Genetics, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium.
6
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
7
School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada.
8
Department of Quantitative Methods, Universidad Loyola Andalucia, Sevilla, Spain.

Abstract

In vitro models of postimplantation human development are valuable to the fields of regenerative medicine and developmental biology. Here, we report characterization of a robust in vitro platform that enabled high-content screening of multiple human pluripotent stem cell (hPSC) lines for their ability to undergo peri-gastrulation-like fate patterning upon bone morphogenetic protein 4 (BMP4) treatment of geometrically confined colonies and observed significant heterogeneity in their differentiation propensities along a gastrulation associable and neuralization associable axis. This cell line-associated heterogeneity was found to be attributable to endogenous Nodal expression, with up-regulation of Nodal correlated with expression of a gastrulation-associated gene profile, and Nodal down-regulation correlated with a preneurulation-associated gene profile expression. We harness this knowledge to establish a platform of preneurulation-like fate patterning in geometrically confined hPSC colonies in which fates arise because of a BMPs signalling gradient conveying positional information. Our work identifies a Nodal signalling-dependent switch in peri-gastrulation versus preneurulation-associated fate patterning in hPSC cells, provides a technology to robustly assay hPSC differentiation outcomes, and suggests conserved mechanisms of organized fate specification in differentiating epiblast and ectodermal tissues.

Conflict of interest statement

The authors have declared that no competing interests exist.

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