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Syst Biol. 2019 Oct 21. pii: syz068. doi: 10.1093/sysbio/syz068. [Epub ahead of print]

Phylogenomic Resolution of the Cetacean Tree of Life Using Target Sequence Capture.

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School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK.
Department of Vertebrate Zoology, Smithsonian National Museum of Natural History, Washington, DC, USA.
Institute of Zoology, Zoological Society of London, London, UK.
School of Biological Sciences, University of Western Australia, Perth WA 6009, Australia.
Australian Antarctic Division, Kingston, Tasmania, Australia.
Southwest Fisheries Science Center, National Marine Fisheries Service, NOAA, La Jolla, CA, USA.


The evolution of cetaceans, from their early transition to an aquatic lifestyle to their subsequent diversification, has been the subject of numerous studies. However, while the higher-level relationships among cetacean families have been largely settled, several aspects of the systematics within these groups remain unresolved. Problematic clades include the oceanic dolphins (37 spp.), which have experienced a recent rapid radiation, and the beaked whales (22 spp.), which have not been investigated in detail using nuclear loci. The combined application of high-throughput sequencing with techniques that target specific genomic sequences provide a powerful means of rapidly generating large volumes of orthologous sequence data for use in phylogenomic studies. To elucidate the phylogenetic relationships within the Cetacea, we combined sequence capture with Illumina sequencing to generate data for ∼3200 protein-coding genes for 68 cetacean species and their close relatives including the pygmy hippopotamus. By combining data from >38,000 exons with existing sequences from 11 cetaceans and seven outgroup taxa, we produced the first comprehensive comparative genomic dataset for cetaceans, spanning 6,527,596 aligned base pairs and 89 taxa. Phylogenetic trees reconstructed with maximum likelihood and Bayesian inference of concatenated loci, as well as with coalescence analyses of individual gene trees, produced mostly concordant and well-supported trees. Our results completely resolve the relationships among beaked whales as well as the contentious relationships among oceanic dolphins, especially the problematic subfamily Delphininae. We carried out Bayesian estimation of species divergence times using MCMCTree, and compared our complete dataset to a subset of clocklike genes. Analyses using the complete dataset consistently showed less variance in divergence times than the reduced dataset. In addition, integration of new fossils (e.g., Mystacodon selenensis) indicate that the diversification of Crown Cetacea began before the Late Eocene and the divergence of Crown Delphinidae as early as the Middle Miocene.


Cetaceans; Delphinidae; Ziphiidae; dolphins; phylogenomics; whales


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