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Development. 2019 Nov 14;146(22). pii: dev180091. doi: 10.1242/dev.180091.

Morphogenetic control of zebrafish cardiac looping by Bmp signaling.

Author information

1
Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad Nacional de Rosario, 2000 Rosario, Argentina salim.seyfried@uni-potsdam.de lombardo@ibr-conicet.gov.ar.
2
Centro de Estudios Interdisciplinarios, Universidad Nacional de Rosario, 2000 Rosario, Argentina.
3
Institute of Molecular Biology, Hannover Medical School, D-30625 Hannover, Germany.
4
Institute of Biochemistry and Biology, Potsdam University, D-14476 Potsdam, Germany.
5
Institute of Anatomy and Embryology, UMG, Göttingen University, D-37075 Göttingen, Germany.
6
Institute of Molecular Biology, Hannover Medical School, D-30625 Hannover, Germany salim.seyfried@uni-potsdam.de lombardo@ibr-conicet.gov.ar.

Abstract

Cardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire a conical cell shape, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of bone morphogenetic protein signaling, myocardial cells at the superior wall of the atrioventricular canal maintain cuboidal cell shapes and S-shaped bending is impaired. This description of cellular rearrangements and cardiac looping regulation may also be relevant for understanding the etiology of human congenital heart defects.

KEYWORDS:

BMP; Cardiac looping; Hemodynamics; Wnt; Zebrafish

PMID:
31628109
DOI:
10.1242/dev.180091

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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